Live-Ptychography: How to Solve a Quantitative Live Object Transfer Function in Microscopy?

In transmission electron microscopy we record the intensity of an electron wave that has been created by the illumination optics, transmitted through a thin specimen, and then projected onto a detector. From that recorded intensity we want to learn about the specimen. Ptychography is a method to reconstruct a quantitative object transfer function from a set of recorded intensities. The illumination is spatially modulated and shifted between measurements to obtain additional information which allows solving for the transfer function of the specimen. Doing this on live data streams combines challenges from different areas: On the one hand, it requires a suitable mathematical approach to solve for the transfer function from a dynamically growing set of intensity measurements while providing intermediate results for live visualization. On the other hand, it requires engineering to reach the required throughput and integrate with real-work data sources, experiments and users. This seminar gives a short introduction to two approaches for live ptychography as well as the surrounding engineering.